Twenty isolates of bacterial isolates were screening for peroxidase activity. The isolate 13 was performance higher peroxidase activity among all isolates with activity (23 U/ml). The selected isolate was characterized and identified according to morphological and biochemical tests as Pseudomonas aeruginosa. The optimum pH for peroxidase activity was 6 with activity (42) U/ml. The result showed an increase in the activity at 40°C and peroxidase activity reached to (71) U/ml. The peroxidase from Pseudomonas sp able to degrade aflatoxin B1 after 24h and the maximum degradation (87%) was attained after 72 h at enzyme concentration 71 U/ml.